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\n\n \n \n Sakai, T., Yamamoto, T., Watanabe, T., Hozumi, A., Shiraishi, A., Osugi, T., Matsubara, S., Kawada, T., Sasakura, Y., Takahashi, T., & Satake, H.\n\n\n \n \n \n \n \n Characterization of a novel species-specific 51-amino acid peptide, PEP51, as a caspase-3/7 activator in ovarian follicles of the ascidian, Ciona intestinalis Type A.\n \n \n \n \n\n\n \n\n\n\n
Frontiers in Endocrinology, 14: 1260600. September 2023.\n
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\n\n \n \n ![\"Characterization](\"//bibbase.org/img/filetypes/link.svg\"\n\t "\\"Paper\\"\n\t")
Paper\n \n \n\n \n \n doi\n \n \n\n \n link\n \n \n\n bibtex\n \n\n \n \n \n abstract \n \n\n \n\n \n \n \n \n \n \n \n\n \n \n \n\n\n\n
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@article{sakai_characterization_2023,\n\ttitle = {Characterization of a novel species-specific 51-amino acid peptide, {PEP51}, as a caspase-3/7 activator in ovarian follicles of the ascidian, \\textit{{Ciona} intestinalis} {Type} {A}},\n\tvolume = {14},\n\tissn = {1664-2392},\n\turl = {https://www.frontiersin.org/articles/10.3389/fendo.2023.1260600/full},\n\tdoi = {10.3389/fendo.2023.1260600},\n\tabstract = {Invertebrates lack hypothalamic-pituitary-gonadal axis, and have acquired species-specific regulatory systems for ovarian follicle development. Ascidians are marine invertebrates that are the phylogenetically closest living relatives to vertebrates, and we have thus far substantiated the molecular mechanisms underlying neuropeptidergic follicle development of the cosmopolitan species,\n Ciona intestinalis\n Type A. However, no ovarian factor has so far been identified in\n Ciona\n . In the present study, we identified a novel\n Ciona\n -specific peptide, termed PEP51, in the ovary. Immunohistochemical analysis demonstrated the specific expression of PEP51 in oocyte-associated accessory cells, test cells, of post-vitellogenic (stage III) follicles. Immunoelectron microscopy revealed that PEP51 was localized in the cytosol of test cells in early stage III follicles, which lack secretory granules. These results indicate that PEP51 acts as an intracellular factor within test cells rather than as a secretory peptide. Confocal laser microscopy verified that activation of caspase-3/7, the canonical apoptosis marker, was detected in most PEP51-positive test cells of early stage III. This colocalization of PEP51 and the apoptosis marker was consistent with immunoelectron microscopy observations demonstrating that a few normal (PEP51-negative) test cells reside in the aggregates of PEP51-positive apoptotic test cells of early stage III follicles. Furthermore, transfection of the PEP51 gene into COS-7 cells and HEK293MSR cells resulted in activation of caspase-3/7, providing evidence that PEP51 induces apoptotic signaling. Collectively, these results showed the existence of species-specific ovarian peptide-driven cell metabolism in\n Ciona\n follicle development. Consistent with the phylogenetic position of\n Ciona\n as the closest sister group of vertebrates, the present study sheds new light on the molecular and functional diversity of the regulatory systems of follicle development in the Chordata.},\n\turldate = {2024-04-04},\n\tjournal = {Frontiers in Endocrinology},\n\tauthor = {Sakai, Tsubasa and Yamamoto, Tatsuya and Watanabe, Takehiro and Hozumi, Akiko and Shiraishi, Akira and Osugi, Tomohiro and Matsubara, Shin and Kawada, Tsuyoshi and Sasakura, Yasunori and Takahashi, Toshio and Satake, Honoo},\n\tmonth = sep,\n\tyear = {2023},\n\tpages = {1260600},\n}\n\n\n
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\n Invertebrates lack hypothalamic-pituitary-gonadal axis, and have acquired species-specific regulatory systems for ovarian follicle development. Ascidians are marine invertebrates that are the phylogenetically closest living relatives to vertebrates, and we have thus far substantiated the molecular mechanisms underlying neuropeptidergic follicle development of the cosmopolitan species, Ciona intestinalis Type A. However, no ovarian factor has so far been identified in Ciona . In the present study, we identified a novel Ciona -specific peptide, termed PEP51, in the ovary. Immunohistochemical analysis demonstrated the specific expression of PEP51 in oocyte-associated accessory cells, test cells, of post-vitellogenic (stage III) follicles. Immunoelectron microscopy revealed that PEP51 was localized in the cytosol of test cells in early stage III follicles, which lack secretory granules. These results indicate that PEP51 acts as an intracellular factor within test cells rather than as a secretory peptide. Confocal laser microscopy verified that activation of caspase-3/7, the canonical apoptosis marker, was detected in most PEP51-positive test cells of early stage III. This colocalization of PEP51 and the apoptosis marker was consistent with immunoelectron microscopy observations demonstrating that a few normal (PEP51-negative) test cells reside in the aggregates of PEP51-positive apoptotic test cells of early stage III follicles. Furthermore, transfection of the PEP51 gene into COS-7 cells and HEK293MSR cells resulted in activation of caspase-3/7, providing evidence that PEP51 induces apoptotic signaling. Collectively, these results showed the existence of species-specific ovarian peptide-driven cell metabolism in Ciona follicle development. Consistent with the phylogenetic position of Ciona as the closest sister group of vertebrates, the present study sheds new light on the molecular and functional diversity of the regulatory systems of follicle development in the Chordata.\n
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\n\n \n \n Sasakura, Y., & Horie, T.\n\n\n \n \n \n \n \n Improved Genome Editing in the Ascidian Ciona with CRISPR/Cas9 and TALEN.\n \n \n \n \n\n\n \n\n\n\n In Hatada, I., editor(s),
Genome Editing in Animals, volume 2637, pages 375–388. Springer US, New York, NY, 2023.\n
Series Title: Methods in Molecular Biology\n\n
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@incollection{hatada_improved_2023,\n\taddress = {New York, NY},\n\ttitle = {Improved {Genome} {Editing} in the {Ascidian} \\textit{{Ciona}} with {CRISPR}/{Cas9} and {TALEN}},\n\tvolume = {2637},\n\tisbn = {978-1-07-163015-0 978-1-07-163016-7},\n\turl = {https://link.springer.com/10.1007/978-1-0716-3016-7_28},\n\tlanguage = {en},\n\turldate = {2023-05-11},\n\tbooktitle = {Genome {Editing} in {Animals}},\n\tpublisher = {Springer US},\n\tauthor = {Sasakura, Yasunori and Horie, Takeo},\n\teditor = {Hatada, Izuho},\n\tyear = {2023},\n\tdoi = {10.1007/978-1-0716-3016-7_28},\n\tnote = {Series Title: Methods in Molecular Biology},\n\tpages = {375--388},\n}\n\n\n
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\n\n \n \n Totsuka, N. M., Kuwana, S., Sawai, S., Oka, K., Sasakura, Y., & Hotta, K.\n\n\n \n \n \n \n \n Distribution changes of non‐self‐test cells and self‐tunic cells surrounding the outer body during Ciona metamorphosis.\n \n \n \n \n\n\n \n\n\n\n
Developmental Dynamics, 252(11): 1363–1374. November 2023.\n
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\n\n \n \n Paper\n \n \n\n \n \n doi\n \n \n\n \n link\n \n \n\n bibtex\n \n\n \n \n \n abstract \n \n\n \n\n \n \n \n \n \n \n \n\n \n \n \n\n\n\n
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@article{totsuka_distribution_2023,\n\ttitle = {Distribution changes of non‐self‐test cells and self‐tunic cells surrounding the outer body during \\textit{{Ciona}} metamorphosis},\n\tvolume = {252},\n\tissn = {1058-8388, 1097-0177},\n\turl = {https://anatomypubs.onlinelibrary.wiley.com/doi/10.1002/dvdy.636},\n\tdoi = {10.1002/dvdy.636},\n\tabstract = {Abstract\n \n Background\n : Ascidians significantly change their body structure through metamorphosis, but the spatio‐temporal cell dynamics in the early metamorphosis stage has not been clarified. A natural\n Ciona\n embryo is surrounded by maternally derived non‐self‐test cells before metamorphosis. However, after metamorphosis, the juvenile is surrounded by self‐tunic cells derived from mesenchymal cell lineages. Both test cells and tunic cells are thought to be changed their distributions during metamorphosis, but the precise timing is unknown.\n \n \n Results\n : Using a metamorphosis induction by mechanical stimulation, we investigated the dynamics of mesenchymal cells during metamorphosis in a precise time course. After the stimulation, two‐round Ca\n 2+\n transients were observed. Migrating mesenchymal cells came out through the epidermis within 10 min after the second phase. We named this event “cell extravasation.” The cell extravasation occurred at the same time as the backward movement of posterior trunk epidermal cells. Timelapse imaging of transgenic‐line larva revealed that non‐self‐test cells and self‐tunic cells temporarily coexist outside the body until the test cells are eliminated. At the juvenile stage, only extravasated self‐tunic cells remained outside the body.\n \n \n Conclusions\n : We found that mesenchymal cells extravasated following two‐round Ca\n 2+\n transients, and distributions of test cells and tunic cells changed in the outer body after tail regression.\n \n , \n Key Findings\n \n \n \n We found mesenchymal cells were released from multiple points in the epidermis of the trunk (extravasation) after mechanical stimulation.\n \n \n Extravasation occurs simultaneously with backward movement of the epidermis.\n \n \n Before metamorphosis, mesenchymal cells already migrated beneath the epidermis.\n \n \n Three‐dimensional timelapse imaging revealed that extravasated cells were divided into two groups: faster migrating cell groups than before metamorphosis and slower cell groups.\n \n \n After extravasation, non‐self‐external cells are eliminated and self‐extravasated cells surrounded the outer body. Both cells are thought to be responsible for defense.},\n\tlanguage = {en},\n\tnumber = {11},\n\turldate = {2024-04-04},\n\tjournal = {Developmental Dynamics},\n\tauthor = {Totsuka, Nozomu M. and Kuwana, Satoshi and Sawai, Satoshi and Oka, Kotaro and Sasakura, Yasunori and Hotta, Kohji},\n\tmonth = nov,\n\tyear = {2023},\n\tpages = {1363--1374},\n}\n\n\n
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\n Abstract Background : Ascidians significantly change their body structure through metamorphosis, but the spatio‐temporal cell dynamics in the early metamorphosis stage has not been clarified. A natural Ciona embryo is surrounded by maternally derived non‐self‐test cells before metamorphosis. However, after metamorphosis, the juvenile is surrounded by self‐tunic cells derived from mesenchymal cell lineages. Both test cells and tunic cells are thought to be changed their distributions during metamorphosis, but the precise timing is unknown. Results : Using a metamorphosis induction by mechanical stimulation, we investigated the dynamics of mesenchymal cells during metamorphosis in a precise time course. After the stimulation, two‐round Ca 2+ transients were observed. Migrating mesenchymal cells came out through the epidermis within 10 min after the second phase. We named this event “cell extravasation.” The cell extravasation occurred at the same time as the backward movement of posterior trunk epidermal cells. Timelapse imaging of transgenic‐line larva revealed that non‐self‐test cells and self‐tunic cells temporarily coexist outside the body until the test cells are eliminated. At the juvenile stage, only extravasated self‐tunic cells remained outside the body. Conclusions : We found that mesenchymal cells extravasated following two‐round Ca 2+ transients, and distributions of test cells and tunic cells changed in the outer body after tail regression. , Key Findings We found mesenchymal cells were released from multiple points in the epidermis of the trunk (extravasation) after mechanical stimulation. Extravasation occurs simultaneously with backward movement of the epidermis. Before metamorphosis, mesenchymal cells already migrated beneath the epidermis. Three‐dimensional timelapse imaging revealed that extravasated cells were divided into two groups: faster migrating cell groups than before metamorphosis and slower cell groups. After extravasation, non‐self‐external cells are eliminated and self‐extravasated cells surrounded the outer body. Both cells are thought to be responsible for defense.\n
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\n\n \n \n Treen, N., Konishi, S., Nishida, H., Onuma, T. A., & Sasakura, Y.\n\n\n \n \n \n \n \n Zic-r.b controls cell numbers in Ciona embryos by activating CDKN1B.\n \n \n \n \n\n\n \n\n\n\n
Developmental Biology, 498: 26–34. June 2023.\n
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@article{treen_zic-rb_2023,\n\ttitle = {Zic-r.b controls cell numbers in \\textit{{Ciona}} embryos by activating {CDKN1B}},\n\tvolume = {498},\n\tissn = {00121606},\n\turl = {https://linkinghub.elsevier.com/retrieve/pii/S0012160623000489},\n\tdoi = {10.1016/j.ydbio.2023.03.005},\n\tlanguage = {en},\n\turldate = {2023-05-11},\n\tjournal = {Developmental Biology},\n\tauthor = {Treen, Nicholas and Konishi, Shohei and Nishida, Hiroki and Onuma, Takeshi A. and Sasakura, Yasunori},\n\tmonth = jun,\n\tyear = {2023},\n\tpages = {26--34},\n}\n\n\n
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